ABSTRACT
Objectives: Since the admission of the critically ill American patients from Diamond Princess on February 11, 2020, our hospital has accepted a total of 300 Covid-19 infected patients. Materials and Methods: On January 29, 2020, we established our own PCR test method based on the SARS-CoV-2 genome data (Hirotsu Y, et al. J Virol Methods. 2020). In the PCR test for screening of all hospitalized patients, the minimum detection limit was determined based on the data of positive sample dilution experiments, and a method for testing multiple samples at once was established using the ''Pooling Strategy'' (Hirotsu Y, et al. Sci Rep. 2020). To date, the total number of PCR tests performed at our hospital has exceeded 25,832 cases. In addition, we have established a system that allows us to perform antigen/antibody testing and PCR testing at the same time for urgent hospitalization and emergency surgery cases without interfering with the emergency system. Results: With these systems in place, we have been able to achieve zero nosocomial infections and no staff waiting at home, and have been able to perform surgical operations as usual. Conclusion: Currently, the Genome Analysis Center has resumed genome analysis of the accumulated liver cancer resection specimens. Panel analysis of hepatocarcinoma-associated mutated genes (72 SMGs) using next-generation sequencers has shown a trend toward early recurrence and poor prognosis after hepatic resection in cases of multinodular hepatocarcinoma that share multiple oncogenic mutations.